Dental News - Composition of platelet-rich plasma gel: A Western blot analysis

Dr. Pablo Galindo Moreno et al.

Tue. 23. August 2016

save

Objective: Platelet-rich plasma (PRP) gel is an autogenous blood-derived material that may be used as a regenerative agent of oral structures. The regenerative capacity of PRP is largely attributed to its composition, including many different growth factors. Thus far, no study has identified the molecular content of this gel. Therefore, it was the purpose of this study to assess the presence of different growth factors in PRP gel, using the Western blot technique.

Materials and methods: Blood samples were collected from 20 healthy donors and then processed to obtain PRP gel samples. The Western blot technique was used to determine the presence of the following growth factors: vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF-β1), fibroblast growth factor (FGF), insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF).

Results: Western blot analysis showed positive electrophoretic bands corresponding to molecular weights of the examined platelet growth factors. These bands were observed in every sample of PRP, demonstrating their presence as constituents of PRP gel.

Conclusion: Data from our study showed that PRP gel contains varying amounts of VEGF, TGF-β1, bFGF, PDGF-A, IGF-1 and EGF.

Editorial note: The full article was published in the 2/2016 issue of the Journal of Oral Science and Rehabilitation. Access the full article at www.dtscience.com.

To post a reply please login or register

Researchers examine composition of oral microbiome in children

VANCOUVER, British Columbia, Canada/COLUMBUS, Ohio, U.S.: The composition of the oral microbiome is critically important in oral health and disease, but the...

In vitro analysis of efficiency and safety of a new motion for endodontic instrumentation: TF Adaptive

The introduction of the nickel-titanium (NiTi) alloy in endodontics was a significant improvement, allowing good results in terms of cleaning and shaping of...

Retrospective analysis of periimplantitis therapy of 158 implants

Objective: The objective of the retrospective analysis was to evaluate the efficacy of periimplantitis treatment up to a five-year observation period.

Long-term analysis of primary, non-surgical root canal treatments – A retrospective study

The aim of this study was to exam more than 8,000 primary, non-surgical root canal treatments in the author’s general practice during 1985–1999 ...

Tooth analysis reveals secrets of our earliest ancestors

LISMORE/MELBOURNE, Australia: Extended parental care is considered one of the hallmarks of human evolution. Through tooth chemistry analysis, scientists ...

Interview: “I saw a lot of improvements in every step of the endodontic treatment”

Besides running a private practice concentrating mainly on endodontic dentistry, Dr Filippo Cardinali gives lectures in theoretical and practical courses on...

Benefits of water fluoridation may have peaked in rich countries

MANCHESTER, UK: Researchers from the University of Manchester have conducted a pioneering study in the UK focusing on the oral health and economic impacts ...

Kinesiographic analysis of lateral excursive movement on the horizontal plane: the retrusive component

The temporomandibular joint (TMJ) has a functionally complex articulation that during phylogeny underwent an adaptation also linked to posture change after ...

Analysis of ancient fossil teeth provides insight into breastfeeding habits

BRISTOL, UK: The importance of mother’s milk to an infant’s development is now well established. In a recent study, researchers analysed the fossilised ...